Virus biologique

Published online Jun Author information Article notes Copyright and License information Disclaimer. Received Nov 27; Accepted May This work is licensed under a Creative Commons Attribution 4. This article has been cited by other articles in PMC.

Results Visualization and efficiency of aerosol sampling as determined by electron microscopy To develop and validate the ionizing technique for collection and identification of viral pathogens, we used several viruses of clinical importance; calicivirus, rotavirus and influenza virus H3N2, strain Salomon Island as well as latex particles.

Open in a separate window. Figure 1. Ionizing air and electrostatic attraction collects aerosol-distributed viruses as determined by RT-qPCR We next determined the capacity of RT-qPCR technology to quantitate the capacity of the ionizer technique to collect and concentrate viruses.

Figure 2. Figure 3. Figure 4. Discussion We describe a simple ionizing device operating at 12 volt that can prevent spread of airborne transmitted viral infections between animals in a controlled setting, whilst simultaneously collecting virus from air for rapid identification. Ionizer technology and device The ionizing device used in this study was developed on the basis of the ion-flow ionizing technology from LightAir AB, Solna, Sweden www.

Scanning electron microscopy SEM Collected samples were added on the surface of a polycarbonate 0. Determination of rotavirus and CaCV infectivity Rotavirus stock and samples were diluted in Eagles MEM and subsequently diluted in two-fold dilutions. Airborne transmission of influenza virus We use a guinea pig animal model to investigate whether the ionizing technique could prevent transmission of influenza virus infection, since this model have successfully been used as a model of aerosol transmission studies of influenza virus 31 , Extraction of influenza RNA from guniea pig tissue RNA was extracted from trachea and lung tissue of infected guinea pig.

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Vet Rec , —, Elisa : enzyme-linked immunosorbent assay ; RT-PCR : reverse transcription polymerase chain reaction. National Center for Biotechnology Information , U. Actual Pharm. DOI : Author information Copyright and License information Disclaimer. All rights reserved. Elsevier hereby grants permission to make all its COVIDrelated research that is available on the COVID resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source.

Abstract Since the emergence of the new Sars-CoV-2 coronavirus in China at the end of December and its spread around the world, the scientific community has been mobilized to study its phylogeny, virological aspects, and to understand viral and immune kinetics. Open in a separate window. Figure 1. Clinical features of patients infected with novel coronavirus in Wuhan, China. Wang D. Clinical characteristics of hospitalized patients with novel coronavirus-infected pneumonia in Wuhan, China.

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Guo L.